3O0X
Structural basis of carbohydrate recognition by calreticulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.619, 43.225, 84.632 |
| Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
| Resolution | 42.080 - 2.010 |
| R-factor | 0.1931 |
| Rwork | 0.191 |
| R-free | 0.24041 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o0w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.217 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.080 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 34462 | |
| Completeness [%] | 100.0 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 25% (w/v) PEG 2000 MME, 0.15 M KSCN, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
