3NYT

X-ray crystal structure of the WlbE (WpbE) aminotransferase from pseudomonas aeruginosa, mutation K185A, in complex with the PLP external aldimine adduct with UDP-3-amino-2-N-acetyl-glucuronic acid, at 1.3 angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Aminotransferase WbpE	polymer	367	39978.6	1	UniProt (Q9HZ76) Pfam (PF01041) In PDB	Pseudomonas aeruginosa
2	A	(2S,3S,4R,5R,6R)-5-(acetylamino)-6-{[(R)-{[(S)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-3-hydroxy-4-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}tetrahydro-2H-pyran-2-carboxylic acid (non-preferred name)	non-polymer		849.5	1	Chemie (ULP)
3	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4		water	water		18.0	470	Chemie (HOH)

Sequence modifications

A: 1 - 359 (UniProt: Q9HZ76)

PDB	External Database	Details
Ala 185	Lys 185	engineered mutation
Leu 360	-	expression tag
Glu 361	-	expression tag
His 362	-	expression tag
His 363	-	expression tag
His 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39978.6
Non-Polymers*	Number of molecules	2
	Total formula weight	872.5
All*	Total formula weight	40851.1

*Water molecules are not included.