3NSJ

The X-ray crystal structure of lymphocyte perforin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Perforin-1	polymer	540	60799.0	1	UniProt (P10820) Pfam (PF01823) Pfam (PF00168) In PDB	Mus musculus (mouse)	P1, Lymphocyte pore-forming protein, Cytolysin
2	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	A	IODIDE ION	non-polymer		126.9	4	Chemie (IOD)
5	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
7		water	water		18.0	279	Chemie (HOH)

Sequence modifications

A: 21 - 554 (UniProt: P10820)

PDB	External Database	Details
Glu 213	Arg 213	engineered mutation
His 555	-	expression tag
His 556	-	expression tag
His 557	-	expression tag
His 558	-	expression tag
His 559	-	expression tag
His 560	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	60799.0
Non-Polymers*	Number of molecules	12
	Total formula weight	1156.2
All*	Total formula weight	61955.1

*Water molecules are not included.