3NIC

DNA binding and cleavage by the GIY-YIG endonuclease R.Eco29kI inactive variant Y49F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Eco29kIR	polymer	235	26794.0	8	UniProt (Q46944) Pfam (PF09517) In PDB	Escherichia coli	Restriction endonuclease
2	M, I, K, O	DNA (5'-D(P*CP*GP*GP*GP*AP*GP*GP*CP*CP*CP*GP*CP*GP*GP*GP*CP*CP*GP*CP*CP*GP*C)-3')	polymer	22	6781.3	4
3	N, J, L, P	DNA (5'-D(P*GP*CP*GP*GP*CP*GP*GP*CP*CP*CP*GP*CP*GP*GP*GP*CP*CP*TP*CP*CP*CP*G)-3')	polymer	22	6732.3	4
4	H, A, B, D, E...	PHOSPHATE ION	non-polymer		95.0	11	Chemie (PO4)
5		water	water		18.0	466	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 2 - 214 (UniProt: Q46944)

PDB	External Database	Details
Met -20	-	expression tag
Ala -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag
Phe 49	Tyr 49	engineered mutation
Lys 69	Leu 69	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	268406.5
Non-Polymers*	Number of molecules	11
	Total formula weight	1044.7
All*	Total formula weight	269451.1

*Water molecules are not included.