3NIC
DNA binding and cleavage by the GIY-YIG endonuclease R.Eco29kI inactive variant Y49F
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E... | Eco29kIR | polymer | 235 | 26794.0 | 8 | UniProt (Q46944) Pfam (PF09517) In PDB | Escherichia coli | Restriction endonuclease |
2 | M, I, K, O | DNA (5'-D(P*CP*GP*GP*GP*AP*GP*GP*CP*CP*CP*GP*CP*GP*GP*GP*CP*CP*GP*CP*CP*GP*C)-3') | polymer | 22 | 6781.3 | 4 | |||
3 | N, J, L, P | DNA (5'-D(P*GP*CP*GP*GP*CP*GP*GP*CP*CP*CP*GP*CP*GP*GP*GP*CP*CP*TP*CP*CP*CP*G)-3') | polymer | 22 | 6732.3 | 4 | |||
4 | H, A, B, D, E... | PHOSPHATE ION | non-polymer | 95.0 | 11 | Chemie (PO4) | |||
5 | water | water | 18.0 | 466 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H: 2 - 214 (UniProt: Q46944)
PDB | External Database | Details |
---|---|---|
Met -20 | - | expression tag |
Ala -19 | - | expression tag |
Ser -18 | - | expression tag |
Ser -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Ser -10 | - | expression tag |
Ser -9 | - | expression tag |
Gly -8 | - | expression tag |
Leu -7 | - | expression tag |
Val -6 | - | expression tag |
Pro -5 | - | expression tag |
Arg -4 | - | expression tag |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Ser -1 | - | expression tag |
Met 0 | - | expression tag |
Gly 1 | - | expression tag |
Phe 49 | Tyr 49 | engineered mutation |
Lys 69 | Leu 69 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 16 |
Total formula weight | 268406.5 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 1044.7 | |
All* | Total formula weight | 269451.1 |