3N9U

Crystal Structure of the Complex between the 25 kDa Subunit and the 59 kDa Subunit (RRM domain) of Human Cleavage Factor Im

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cleavage and polyadenylation specificity factor subunit 5	polymer	230	26720.7	2	UniProt (O43809) Pfam (PF13869)	Homo sapiens (human)	Cleavage and polyadenylation specificity factor 25 kDa subunit, CPSF 25 kDa subunit, Pre-mRNA cleavage factor Im 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21
2	C, D (C, I)	Cleavage and polyadenylation specificity factor subunit 7	polymer	156	17711.0	2	UniProt (Q8N684) Pfam (PF00076)	Homo sapiens (human)	Cleavage and polyadenylation specificity factor 59 kDa subunit, CPSF 59 kDa subunit, Pre-mRNA cleavage factor Im 59 kDa subunit
3	E, F, G (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	H, I, J, K (A, B, C, I)	water	water		18.0	417	Chemie (HOH)

Sequence modifications

A, B: 21 - 227 (UniProt: O43809)

PDB	External Database	Details
Met -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
Ser 5	-	expression tag
Ser 6	-	expression tag
Gly 7	-	expression tag
Val 8	-	expression tag
Asp 9	-	expression tag
Leu 10	-	expression tag
Gly 11	-	expression tag
Thr 12	-	expression tag
Glu 13	-	expression tag
Asn 14	-	expression tag
Leu 15	-	expression tag
Tyr 16	-	expression tag
Phe 17	-	expression tag
Gln 18	-	expression tag
Ser 19	-	expression tag
Met 20	-	expression tag

C, I: 50 - 182 (UniProt: Q8N684)

PDB	External Database	Details
Met 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
Ser 34	-	expression tag
Ser 35	-	expression tag
Gly 36	-	expression tag
Val 37	-	expression tag
Asp 38	-	expression tag
Leu 39	-	expression tag
Gly 40	-	expression tag
Thr 41	-	expression tag
Glu 42	-	expression tag
Asn 43	-	expression tag
Leu 44	-	expression tag
Tyr 45	-	expression tag
Phe 46	-	expression tag
Gln 47	-	expression tag
Ser 48	-	expression tag
Met 49	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	88863.3
Non-Polymers*	Number of molecules	3
	Total formula weight	276.3
All*	Total formula weight	89139.6

*Water molecules are not included.