3N9U
Crystal Structure of the Complex between the 25 kDa Subunit and the 59 kDa Subunit (RRM domain) of Human Cleavage Factor Im
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 165.950, 124.730, 40.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.500 - 1.920 |
| R-factor | 0.1978 |
| Rwork | 0.196 |
| R-free | 0.23134 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cl3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.418 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.317 | 2.029 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.113 | 0.438 |
| Number of reflections | 63481 | |
| <I/σ(I)> | 8 | 2.5 |
| Completeness [%] | 97.9 | 94 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl pH 8.5, 20% ethanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
