3N3B

Ribonucleotide Reductase Dimanganese(II)-NrdF from Escherichia coli in Complex with Reduced NrdI with a Trapped Peroxide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	Ribonucleoside-diphosphate reductase 2 subunit beta	polymer	319	36475.1	2	UniProt (P37146) Pfam (PF00268) In PDB	Escherichia coli	Ribonucleotide reductase 2, R2F protein
2	C, D	Protein nrdI	polymer	153	17216.4	2	UniProt (P0A772) Pfam (PF07972) In PDB	Escherichia coli
3	B, A	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
4	B, A	HYDROGEN PEROXIDE	non-polymer		34.0	2	Chemie (PEO)
5	C, D	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
6		water	water		18.0	162	Chemie (HOH)

Sequence modifications

C, D: 1 - 133 (UniProt: P0A772)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	107382.9
Non-Polymers*	Number of molecules	8
	Total formula weight	1200.5
All*	Total formula weight	108583.4

*Water molecules are not included.