3N3B

Ribonucleotide Reductase Dimanganese(II)-NrdF from Escherichia coli in Complex with Reduced NrdI with a Trapped Peroxide

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004748	molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0005971	cellular_component	ribonucleoside-diphosphate reductase complex
A	0009185	biological_process	ribonucleoside diphosphate metabolic process
A	0009263	biological_process	deoxyribonucleotide biosynthetic process
A	0009265	biological_process	2'-deoxyribonucleotide biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0030145	molecular_function	manganese ion binding
A	0046872	molecular_function	metal ion binding
B	0004748	molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0005971	cellular_component	ribonucleoside-diphosphate reductase complex
B	0009185	biological_process	ribonucleoside diphosphate metabolic process
B	0009263	biological_process	deoxyribonucleotide biosynthetic process
B	0009265	biological_process	2'-deoxyribonucleotide biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0030145	molecular_function	manganese ion binding
B	0046872	molecular_function	metal ion binding
C	0005515	molecular_function	protein binding
C	0010181	molecular_function	FMN binding
C	0036211	biological_process	protein modification process
D	0005515	molecular_function	protein binding
D	0010181	molecular_function	FMN binding
D	0036211	biological_process	protein modification process

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MN B 320

Chain	Residue
B	ASP67
B	GLU98
B	HIS101
B	GLU158
B	GLU192
B	MN321
B	HOH375

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN B 321

Chain	Residue
B	GLU192
B	HIS195
B	MN320
B	HOH381
B	GLU98
B	GLU158

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PEO B 322

Chain	Residue
B	SER159
B	LYS260
B	HOH329
C	ASN83
C	ASN85

---

site_id	AC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE FMN C 134

Chain	Residue
B	ARG25
B	TYR197
C	SER8
C	SER9
C	SER11
C	GLU12
C	ASN13
C	THR14
C	PRO47
C	SER48
C	TYR49
C	GLY50
C	GLY51
C	GLY82
C	ASN83
C	PHE86
C	ALA89
C	TYR90
C	GLY91
C	LEU111
C	HOH138
C	HOH143

---

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN A 320

Chain	Residue
A	ASP67
A	GLU98
A	HIS101
A	GLU158
A	GLU192
A	HOH361

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MN A 321

Chain	Residue
A	GLU98
A	GLU158
A	GLU192
A	HIS195
A	HOH334

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PEO A 322

Chain	Residue
A	SER159
A	LYS260
A	ASN264
A	HOH359
D	ASN83
D	ASN85

---

site_id	AC8
Number of Residues	21
Details	BINDING SITE FOR RESIDUE FMN D 134

Chain	Residue
A	ARG25
A	TYR197
D	SER8
D	SER9
D	SER11
D	GLU12
D	ASN13
D	THR14
D	PRO47
D	SER48
D	TYR49
D	GLY50
D	SER81
D	GLY82
D	ASN83
D	PHE86
D	ALA89
D	TYR90
D	GLY91
D	LEU111
D	HOH136

---

Functional Information from PROSITE/UniProt

site_id	PS00368
Number of Residues	17
Details	RIBORED_SMALL Ribonucleotide reductase small subunit signature. MEa.VHArSYssIfstLC

Chain	Residue	Details
B	MET97-CYS113

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000255|PROSITE-ProRule:PRU10014

Chain	Residue	Details
B	TYR105
A	TYR105

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU10014

Chain	Residue	Details
B	ASP67
B	HIS101
A	ASP67
A	HIS101

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
B	GLU98
B	GLU158
A	GLU98
A	GLU158

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING:

Chain	Residue	Details
B	GLU192
B	HIS195
A	GLU192
A	HIS195

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