3MZW

HER2 extracelluar region with affinity matured 3-helix affibody ZHER2:342

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Tyrosine kinase-type cell surface receptor HER2	polymer	624	68794.1	1	UniProt (P04626) Pfam (PF01030) Pfam (PF00757) Pfam (PF14843) In PDB	Homo sapiens (human)	Receptor tyrosine-protein kinase erbB-2, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, Metastatic lymph node gene 19 protein, MLN 19
2	B	Immunoglobulin G-binding protein A	polymer	58	6714.6	1	UniProt (P38507) Pfam (PF02216) In PDB	Staphylococcus aureus	IgG-binding protein A, Staphylococcal protein A
3	C	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5		water	water		18.0	9	Chemie (HOH)

Sequence modifications

B: 1 - 58 (UniProt: P38507)

PDB	External Database	Details
Val 1	Ala 212	engineered mutation
Met 9	Gln 220	engineered mutation
Arg 10	Gln 221	engineered mutation
Tyr 13	Phe 224	engineered mutation
Trp 14	Tyr 225	engineered mutation
Ala 17	Leu 228	engineered mutation
Leu 18	His 229	engineered mutation
Asn 24	Glu 235	engineered mutation
Gln 25	Glu 236	engineered mutation
Lys 27	Arg 238	engineered mutation
Arg 28	Asn 239	engineered mutation
Ala 29	Gly 240	engineered mutation
Arg 32	Gln 243	engineered mutation
Tyr 35	Lys 246	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	75508.6
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	76375.5

*Water molecules are not included.