3MX4

DNA binding and cleavage by the GIY-YIG endonuclease R.Eco29KI inactive variant E142Q

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Eco29kIR	polymer	235	26809.0	8	UniProt (Q46944) Pfam (PF09517) In PDB	Escherichia coli	Restriction endonuclease
2	I, K, M, O	DNA (5'-D(P*CP*GP*GP*GP*AP*GP*GP*CP*CP*CP*GP*CP*GP*GP*GP*CP*CP*GP*CP*CP*GP*C)-3')	polymer	22	6781.3	4
3	J, L, N, P	DNA (5'-D(P*GP*CP*GP*GP*CP*GP*GP*CP*CP*CP*GP*CP*GP*GP*GP*CP*CP*TP*CP*CP*CP*G)-3')	polymer	22	6732.3	4
4		water	water		18.0	493	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 2 - 214 (UniProt: Q46944)

PDB	External Database	Details
Met -20	-	expression tag
Ala -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag
Gly 1	-	expression tag
Lys 69	Leu 69	engineered mutation
Gln 142	Glu 142	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	268526.6
All*	Total formula weight	268526.6

*Water molecules are not included.