3MX4
DNA binding and cleavage by the GIY-YIG endonuclease R.Eco29KI inactive variant E142Q
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 170 |
Detector technology | CCD |
Collection date | 2010-04-20 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 99.855, 101.463, 144.403 |
Unit cell angles | 90.00, 110.19, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.21408 |
Rwork | 0.211 |
R-free | 0.27368 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Apo R.Eco29KI |
RMSD bond length | 0.019 |
RMSD bond angle | 2.101 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 135.530 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.064 | 0.264 |
Number of reflections | 88621 | |
<I/σ(I)> | 19.31 | 6.35 |
Completeness [%] | 99.5 | 100 |
Redundancy | 6.6 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES sodium, 0.8M sodium phosphate monobasic, 0.8M potassium phosphate monobasic, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | HEPES sodium | ||
2 | 1 | 1 | sodium phosphate monobasic | ||
3 | 1 | 1 | potassium phosphate monobasic | ||
4 | 1 | 2 | HEPES sodium | ||
5 | 1 | 2 | sodium phosphate monobasic | ||
6 | 1 | 2 | potassium phosphate monobasic |