3MUZ

E.Coli (lacZ) beta-galactosidase (R599A) in complex with IPTG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	1, 2, 3, 4	Beta-galactosidase	polymer	1052	119657.6	4	UniProt (B8LFD6) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) UniProt (by SIFTS) (P00722) In PDB	Escherichia coli
2	1, 2, 4, 3	1-methylethyl 1-thio-beta-D-galactopyranoside	non-polymer		238.3	8	Chemie (IPT)
3	2, 3, 4, 1	MAGNESIUM ION	non-polymer		24.3	14	Chemie (MG)
4	2, 4, 3, 1	SODIUM ION	non-polymer		23.0	16	Chemie (NA)
5	1, 2, 3, 4	DIMETHYL SULFOXIDE	non-polymer		78.1	150	Chemie (DMS)
6		water	water		18.0	4108	Chemie (HOH)

Sequence modifications

1, 2, 3, 4: 9 - 1023 (UniProt: B8LFD6)

PDB	External Database	Details
Met -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Ser -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Gly -18	-	expression tag
Met -17	-	expression tag
Ala -16	-	expression tag
Ser -15	-	expression tag
Met -14	-	expression tag
Thr -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Gln -10	-	expression tag
Gln -9	-	expression tag
Met -8	-	expression tag
Gly -7	-	expression tag
Arg -6	-	expression tag
Asp -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Asp 1	-	expression tag
Lys 2	-	expression tag
Asp 3	-	expression tag
Pro 4	-	expression tag
Met 5	-	expression tag
Ile 6	-	expression tag
Asp 7	-	expression tag
Pro 8	-	expression tag
Ala 599	Arg 600	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	478630.5
Non-Polymers*	Number of molecules	188
	Total formula weight	14334.5
All*	Total formula weight	492965.0

*Water molecules are not included.