3MUZ
E.Coli (lacZ) beta-galactosidase (R599A) in complex with IPTG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (1, 2, 3, 4) | Beta-galactosidase | polymer | 1052 | 119657.6 | 4 | UniProt (B8LFD6) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) UniProt (by SIFTS) (P00722) | Escherichia coli | |
| 2 | E, F, VA, WA, WE... (1, 2, 4, 3) | 1-methylethyl 1-thio-beta-D-galactopyranoside | non-polymer | 238.3 | 8 | Chemie (IPT) | |||
| 3 | AB, AD, AF, BD, G... (2, 3, 4, 1) | MAGNESIUM ION | non-polymer | 24.3 | 14 | Chemie (MG) | |||
| 4 | BB, BF, CB, CD, CF... (2, 4, 3, 1) | SODIUM ION | non-polymer | 23.0 | 16 | Chemie (NA) | |||
| 5 | AA, AC, AE, AG, BA... (1, 2, 3, 4) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 150 | Chemie (DMS) | |||
| 6 | KG, LG, MG, NG (1, 2, 3, 4) | water | water | 18.0 | 4108 | Chemie (HOH) |
Sequence modifications
1, 2, 3, 4: 9 - 1023 (UniProt: B8LFD6)
| PDB | External Database | Details |
|---|---|---|
| Met -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Ser -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Met -17 | - | expression tag |
| Ala -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Met -14 | - | expression tag |
| Thr -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Gln -10 | - | expression tag |
| Gln -9 | - | expression tag |
| Met -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Arg -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Asp 1 | - | expression tag |
| Lys 2 | - | expression tag |
| Asp 3 | - | expression tag |
| Pro 4 | - | expression tag |
| Met 5 | - | expression tag |
| Ile 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Pro 8 | - | expression tag |
| Ala 599 | Arg 600 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 478630.5 | |
| Non-Polymers* | Number of molecules | 188 |
| Total formula weight | 14334.5 | |
| All* | Total formula weight | 492965.0 |
