3MN6

Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, F, K)	Actin-5C	polymer	374	41723.5	3	UniProt (P10987) Pfam (PF00022)	Drosophila melanogaster (Fruit fly)
2	D, E, F (X, Y, Z)	Protein spire	polymer	19	1635.0	3	UniProt (Q9U1K1)	Drosophila melanogaster (Fruit fly)
3	G, I, K (A, F, K)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
4	H, J, L (A, F, K)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	3	Chemie (ATP)
5	M, N, O, P, Q... (A, F, K, X, Y...)	water	water		18.0	780	Chemie (HOH)

Sequence modifications

A, F, K: 2 - 375 (UniProt: P10987)

PDB	External Database	Details
Glu 204	Ala 205	engineered mutation
Lys 243	Pro 244	engineered mutation

X, Y, Z: 1 - 19 (UniProt: Q9U1K1)

PDB	External Database	Details
Unk 1	Leu 397	SEE REMARK 999
Unk 2	Thr 398	SEE REMARK 999
Unk 3	Pro 399	SEE REMARK 999
Unk 4	Tyr 400	SEE REMARK 999
Unk 5	Glu 401	SEE REMARK 999
Unk 6	Ile 402	SEE REMARK 999
Unk 7	Leu 403	SEE REMARK 999
Unk 8	Met 404	SEE REMARK 999
Unk 9	Gly 405	SEE REMARK 999
Unk 10	Asp 406	SEE REMARK 999
Unk 11	Ile 407	SEE REMARK 999
Unk 12	Arg 408	SEE REMARK 999
Unk 13	Ala 409	SEE REMARK 999
Unk 14	Lys 410	SEE REMARK 999
Unk 15	Lys 411	SEE REMARK 999
Unk 16	Tyr 412	SEE REMARK 999
Unk 17	Gln 413	SEE REMARK 999
Unk 18	Leu 414	SEE REMARK 999
Unk 19	Arg 415	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	130075.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1641.8
All*	Total formula weight	131717.4

*Water molecules are not included.