3MMV

Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Actin-5C	polymer	374	41723.5	1	UniProt (P10987) Pfam (PF00022) In PDB	Drosophila melanogaster (Fruit fly)
2	X	Protein spire	polymer	19	1635.0	1	UniProt (Q9U1K1) In PDB	Drosophila melanogaster (Fruit fly)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
5		water	water		18.0	30	Chemie (HOH)

Sequence modifications

A: 2 - 375 (UniProt: P10987)

PDB	External Database	Details
Glu 204	Ala 205	engineered mutation
Lys 243	Pro 244	engineered mutation

X: 1 - 19 (UniProt: Q9U1K1)

PDB	External Database	Details
Unk 1	Lys 429	SEE REMARK 999
Unk 2	Asp 430	SEE REMARK 999
Unk 3	Ala 431	SEE REMARK 999
Unk 4	His 432	SEE REMARK 999
Unk 5	Ala 433	SEE REMARK 999
Unk 6	Met 434	SEE REMARK 999
Unk 7	Ile 435	SEE REMARK 999
Unk 8	Leu 436	SEE REMARK 999
Unk 9	Glu 437	SEE REMARK 999
Unk 10	Phe 438	SEE REMARK 999
Unk 11	Ile 439	SEE REMARK 999
Unk 12	Arg 440	SEE REMARK 999
Unk 13	Ser 441	SEE REMARK 999
Unk 14	Arg 442	SEE REMARK 999
Unk 15	Pro 443	SEE REMARK 999
Unk 16	Pro 444	SEE REMARK 999
Unk 17	Leu 445	SEE REMARK 999
Unk 18	Lys 446	SEE REMARK 999
Unk 19	Lys 447	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43358.5
Non-Polymers*	Number of molecules	2
	Total formula weight	547.3
All*	Total formula weight	43905.8

*Water molecules are not included.