3MMV
Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-02-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98010 |
| Spacegroup name | P 65 |
| Unit cell lengths | 125.510, 125.510, 56.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.24531 |
| Rwork | 0.241 |
| R-free | 0.32972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hf4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.535 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.086 | 0.297 |
| Number of reflections | 11584 | |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 73.6 | 40.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2 M Ammonium sulphate 0.1 M MES pH 6.5 20%PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
