3MKK
The crystal structure of the D307A mutant of glycoside HYDROLASE (FAMILY 31) from Ruminococcus obeum ATCC 29174 in complex with isomaltose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | alpha-glucosidase GH31 family | polymer | 666 | 77405.5 | 2 | UniProt (A5ZY13) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365) | Ruminococcus obeum | |
| 2 | C (C) | alpha-D-glucopyranose-(1-6)-beta-D-glucopyranose | branched | 342.3 | 1 | In PDB GlyTouCan (G45624LJ) | |||
| 3 | D (A) | alpha-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (GLC) | |||
| 4 | E (A) | beta-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (BGC) | |||
| 5 | F, G (A, B) | water | water | 18.0 | 907 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 663 (UniProt: A5ZY13)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ala 307 | Asp 307 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 154810.9 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 360.3 | |
| All* | Total formula weight | 155513.5 |
