3MIP

I-MsoI re-designed for altered DNA cleavage specificity (-8GCG)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Mso-8G	polymer	161	18713.6	2		synthetic construct (artificial gene)
2	C (C)	DNA (5'-D(*GP*CP*AP*GP*GP*CP*GP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*CP*GP*CP*TP*CP*CP*G)-3')	polymer	24	7412.8	1
3	D (D)	DNA (5'-D(*CP*GP*GP*AP*GP*CP*GP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*CP*GP*CP*CP*TP*GP*C)-3')	polymer	24	7332.7	1
4	E, F, G (A, B, D)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
5	H, I, J, K (A, B, C, D)	water	water		18.0	87	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52172.6
Non-Polymers*	Number of molecules	3
	Total formula weight	120.2
All*	Total formula weight	52292.9

*Water molecules are not included.