3MIP
I-MsoI re-designed for altered DNA cleavage specificity (-8GCG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2008-08-01 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 42.539, 42.611, 71.524 |
Unit cell angles | 96.45, 107.04, 108.56 |
Refinement procedure
Resolution | 22.959 - 2.400 |
R-factor | 0.2316 |
Rwork | 0.226 |
R-free | 0.28550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.184 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.054 | 0.178 |
Number of reflections | 17356 | |
<I/σ(I)> | 5.6 | |
Completeness [%] | 96.7 | 95.1 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 20% PEG 4000, 100mM Tris, 20mM NaCl, 5mM CaCl2, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |