3MFH

DNA Polymerase Eta in Complex With Undamaged DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase eta	polymer	520	58813.4	1	UniProt (Q04049) Pfam (PF00817) Pfam (PF11799)	Saccharomyces cerevisiae (yeast)	Radiation-sensitive protein 30
2	B (P)	5'-D(*GP*TP*CP*CP*TP*CP*CP*CP*CP*TP*(DOC))-3'	polymer	11	3205.1	1
3	C (T)	5'-D(*TP*AP*AP*TP*TP*GP*AP*GP*GP*GP*GP*AP*GP*GP*AP*C)-3'	polymer	16	5027.3	1
4	D (A)	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	1	Chemie (DTP)
5	E, F (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
6	G, H (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	I, J, K (A, P, T)	water	water		18.0	627	Chemie (HOH)

Sequence modifications

A: 1 - 513 (UniProt: Q04049)

PDB	External Database	Details
Gly -6	-	expression tag
Pro -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Ala 140	Lys 140	engineered mutation
Trp 144	Ser 144	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	67045.8
Non-Polymers*	Number of molecules	5
	Total formula weight	731.9
All*	Total formula weight	67777.7

*Water molecules are not included.