3MC6

Crystal structure of ScDPL1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Sphingosine-1-phosphate lyase	polymer	497	54904.5	1	UniProt (Q05567) Pfam (PF00282)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	SP-lyase, ySPL, Sphingosine-1-phosphate aldolase, Bestowed of sphingosine tolerance 1
2	B (C)	Sphingosine-1-phosphate lyase	polymer	497	54676.4	1	UniProt (Q05567) Pfam (PF00282)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	SP-lyase, ySPL, Sphingosine-1-phosphate aldolase, Bestowed of sphingosine tolerance 1
3	C, D, E (A, C)	PHOSPHATE ION	non-polymer		95.0	3	Chemie (PO4)
4	F, G (A, C)	water	water		18.0	4	Chemie (HOH)

Sequence modifications

A: 103 - 589 (UniProt: Q05567)

PDB	External Database	Details
Met 101	-	initiating methionine
Gly 102	-	expression tag
Val 308	Ile 308	engineered mutation
Asp 469	Asn 469	engineered mutation
Leu 590	-	expression tag
Glu 591	-	expression tag
His 592	-	expression tag
His 593	-	expression tag
His 594	-	expression tag
His 595	-	expression tag
His 596	-	expression tag
His 597	-	expression tag

C: 103 - 589 (UniProt: Q05567)

PDB	External Database	Details
Met 101	-	initiating methionine
Gly 102	-	expression tag
Val 308	Ile 308	engineered mutation
Asp 469	Asn 469	engineered mutation
Leu 590	-	expression tag
Glu 591	-	expression tag
His 592	-	expression tag
His 593	-	expression tag
His 594	-	expression tag
His 595	-	expression tag
His 596	-	expression tag
His 597	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	109581.0
Non-Polymers*	Number of molecules	3
	Total formula weight	284.9
All*	Total formula weight	109865.9

*Water molecules are not included.