3MAS
Crystal structure of heterodimeric R132H mutant of human cytosolic NADP(+)-dependent isocitrate dehydrogenase in complex with NADP and isocitrate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Isocitrate dehydrogenase [NADP] cytoplasmic | polymer | 422 | 47772.3 | 1 | UniProt (O75874) Pfam (PF00180) In PDB | Homo sapiens (human) | IDH, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, IDP |
2 | B | Isocitrate dehydrogenase [NADP] cytoplasmic | polymer | 419 | 47237.8 | 1 | UniProt (O75874) Pfam (PF00180) In PDB | Homo sapiens (human) | IDH, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, IDP |
3 | A, B | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 2 | Chemie (NAP) | |||
4 | A, B | ISOCITRIC ACID | non-polymer | 192.1 | 2 | Chemie (ICT) |
Sequence modifications
A: 1 - 414 (UniProt: O75874)
B: 1 - 414 (UniProt: O75874)
PDB | External Database | Details |
---|---|---|
His 132 | Arg 132 | engineered mutation |
Leu 415 | - | expression tag |
Glu 416 | - | expression tag |
His 417 | - | expression tag |
His 418 | - | expression tag |
His 419 | - | expression tag |
His 420 | - | expression tag |
His 421 | - | expression tag |
His 422 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly -4 | - | expression tag |
Ser -3 | - | expression tag |
Pro -2 | - | expression tag |
Glu -1 | - | expression tag |
Phe 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 95010.1 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 1871.1 | |
All* | Total formula weight | 96881.1 |