3MAP

Crystal structure of homodimeric R132H mutant of human cytosolic NADP(+)-dependent isocitrate dehydrogenase in complex with NADP and isocitrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Isocitrate dehydrogenase [NADP] cytoplasmic	polymer	422	47772.3	2	UniProt (O75874) Pfam (PF00180) In PDB	Homo sapiens (human)	IDH, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, IDP
2	A, B	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	2	Chemie (NAP)
3	A, B	ISOCITRIC ACID	non-polymer		192.1	2	Chemie (ICT)

Sequence modifications

A, B: 1 - 414 (UniProt: O75874)

PDB	External Database	Details
His 132	Arg 132	engineered mutation
Leu 415	-	expression tag
Glu 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95544.6
Non-Polymers*	Number of molecules	4
	Total formula weight	1871.1
All*	Total formula weight	97415.7

*Water molecules are not included.