3M63

Crystal structure of Ufd2 in complex with the ubiquitin-like (UBL) domain of Dsk2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin conjugation factor E4	polymer	968	110806.3	1	UniProt (P54860) Pfam (PF10408) Pfam (PF04564) In PDB	Saccharomyces cerevisiae (yeast)	Ubiquitin fusion degradation protein 2, UB fusion protein 2
2	B	Ubiquitin domain-containing protein DSK2	polymer	101	11540.0	1	UniProt (P48510) Pfam (PF00240) In PDB	Saccharomyces cerevisiae (yeast)
3	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
4	A	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
5		water	water		18.0	183	Chemie (HOH)

Sequence modifications

A: 1 - 961 (UniProt: P54860)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Pro -4	-	expression tag
Glu -3	-	expression tag
Phe -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag
Leu 102	Ser 102	engineered mutation
Val 677	Asp 677	engineered mutation

B: 1 - 75 (UniProt: P48510)

PDB	External Database	Details
Met -25	-	expression tag
Lys -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Pro -17	-	expression tag
Met -16	-	expression tag
Ser -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	122346.3
Non-Polymers*	Number of molecules	2
	Total formula weight	277.4
All*	Total formula weight	122623.7

*Water molecules are not included.