3LXC

Interconversion of Human Lysosomal Enzyme Specificities

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Alpha-galactosidase A	polymer	404	46139.3	2	UniProt (P06280) Pfam (PF16499) Pfam (PF17450)	Homo sapiens (human)	Alpha-D-galactoside galactohydrolase, Alpha-D-galactosidase A, Melibiase
2	C, F (C, F)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G81315DD)
3	D (D)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		894.8	1	In PDB GlyTouCan (G17689EW)
4	E (E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
5	G, I (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
6	H, J (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7	K, L (A, B)	water	water		18.0	552	Chemie (HOH)

Sequence modifications

A, B: 32 - 429 (UniProt: P06280)

PDB	External Database	Details
Ser 203	Glu 203	engineered mutation
Ala 206	Leu 206	engineered mutation
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	92278.6
Branched	Number of molecules	4
	Total formula weight	2978.7
Non-Polymers*	Number of molecules	4
	Total formula weight	626.6
All*	Total formula weight	95883.9

*Water molecules are not included.