3LRM

Structure of alfa-galactosidase from Saccharomyces cerevisiae with raffinose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Alpha-galactosidase 1	polymer	479	53083.6	4	UniProt (P04824) Pfam (PF16499) Pfam (PF17801) In PDB	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Alpha-D-galactoside galactohydrolase 1, Melibiase 1
2	E, G, I	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G79924RM)
3	F, H, J	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
4	K, L, M, N	beta-D-fructofuranose-(2-1)-[alpha-D-galactopyranose-(1-6)]alpha-D-glucopyranose	branched		504.4	4	In PDB
5	A, B, C, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	9	Chemie (NAG)
6		water	water		18.0	1022	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 471 (UniProt: P04824)

PDB	External Database	Details
Ala 149	Asp 149	engineered mutation
Val 181	Ile 181	engineered mutation
Asp 472	-	expression tag
Tyr 473	-	expression tag
Lys 474	-	expression tag
Asp 475	-	expression tag
Asp 476	-	expression tag
Asp 477	-	expression tag
Asp 478	-	expression tag
Lys 479	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	212334.5
Branched	Number of molecules	10
	Total formula weight	4564.2
Non-Polymers*	Number of molecules	9
	Total formula weight	1990.9
All*	Total formula weight	218889.5

*Water molecules are not included.