3LRM
Structure of alfa-galactosidase from Saccharomyces cerevisiae with raffinose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0722 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.170, 129.530, 136.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.050 - 2.700 |
| R-factor | 0.243 |
| Rwork | 0.241 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.057 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.050 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.081 | 0.438 |
| Number of reflections | 54965 | |
| <I/σ(I)> | 8.9 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | Deglycosylated sample. 19% PEG3350, 0.1 M BisTris, 0.2 M SCNK, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
