3LOJ

Structure of Mycobacterium tuberculosis dUTPase H145A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Deoxyuridine 5'-triphosphate nucleotidohydrolase	polymer	174	17925.2	1	UniProt (P0A552) Pfam (PF00692) UniProt (by SIFTS) (P9WNS5)	Mycobacterium tuberculosis	dUTPase, dUTP pyrophosphatase
2	B (A)	2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DUP)
3	C, D (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	E (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
5	F (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	G (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
7	H (A)	water	water		18.0	198	Chemie (HOH)

Sequence modifications

A: 1 - 154 (UniProt: P0A552)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 145	His 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	17925.2
Non-Polymers*	Number of molecules	6
	Total formula weight	852.7
All*	Total formula weight	18778.0

*Water molecules are not included.