3LNM

F233W mutant of the Kv2.1 paddle-Kv1.2 chimera channel

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Voltage-gated potassium channel subunit beta-2	polymer	333	37353.1	2	UniProt (P62483) Pfam (PF00248) In PDB	Rattus norvegicus (brown rat rat rats)	K(+) channel subunit beta-2, Kv-beta-2
2	B, D	F233W mutant of the Kv2.1 paddle-Kv1.2 chimera	polymer	514	58860.7	2	UniProt (P63142) UniProt (P15387) Pfam (PF02214) Pfam (PF00520) In PDB	Rattus norvegicus (brown rat rat rats)	Voltage-gated potassium channel subunit Kv1.2, RBK2, RCK5, RAK
3	A, C	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	2	Chemie (NAP)
4	B, D	(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate	non-polymer		749.0	13	Chemie (PGW)
5	D, B	POTASSIUM ION	non-polymer		39.1	10	Chemie (K)
6		water	water		18.0	243	Chemie (HOH)

Sequence modifications

A, C: 36 - 367 (UniProt: P62483)

PDB	External Database	Details
Met 35	-	initiating methionine

B, D: 1 - 266 (UniProt: P63142)

PDB	External Database	Details
Met -18	-	expression tag
Ala -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 31	Cys 31	engineered mutation
Ser 32	Cys 32	engineered mutation
Gln 207	Asn 207	engineered mutation
Trp 233	Phe 233	engineered mutation

B, D: 267 - 298 (UniProt: P15387)
B, D: 299 - 495 (UniProt: P63142)

PDB	External Database	Details
Ser 431	Cys 435	engineered mutation
Ser 478	Cys 482	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	192427.6
Non-Polymers*	Number of molecules	25
	Total formula weight	11614.9
All*	Total formula weight	204042.5

*Water molecules are not included.