3LNM
F233W mutant of the Kv2.1 paddle-Kv1.2 chimera channel
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0002244 | biological_process | hematopoietic progenitor cell differentiation |
| A | 0004033 | molecular_function | aldo-keto reductase (NADPH) activity |
| A | 0005249 | molecular_function | voltage-gated potassium channel activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0005874 | cellular_component | microtubule |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006813 | biological_process | potassium ion transport |
| A | 0008076 | cellular_component | voltage-gated potassium channel complex |
| A | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| A | 0014069 | cellular_component | postsynaptic density |
| A | 0015459 | molecular_function | potassium channel regulator activity |
| A | 0016020 | cellular_component | membrane |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0030424 | cellular_component | axon |
| A | 0034705 | cellular_component | potassium channel complex |
| A | 0043005 | cellular_component | neuron projection |
| A | 0043194 | cellular_component | axon initial segment |
| A | 0043679 | cellular_component | axon terminus |
| A | 0044224 | cellular_component | juxtaparanode region of axon |
| A | 0044325 | molecular_function | transmembrane transporter binding |
| A | 0044877 | molecular_function | protein-containing complex binding |
| A | 0045202 | cellular_component | synapse |
| A | 0045445 | biological_process | myoblast differentiation |
| A | 0050905 | biological_process | neuromuscular process |
| A | 0055085 | biological_process | transmembrane transport |
| A | 0055114 | biological_process | obsolete oxidation-reduction process |
| A | 0070995 | biological_process | NADPH oxidation |
| A | 0071805 | biological_process | potassium ion transmembrane transport |
| A | 0098839 | cellular_component | postsynaptic density membrane |
| A | 0098900 | biological_process | regulation of action potential |
| A | 0098978 | cellular_component | glutamatergic synapse |
| A | 1901379 | biological_process | regulation of potassium ion transmembrane transport |
| A | 1990002 | molecular_function | methylglyoxal reductase (NADPH) (acetol producing) activity |
| A | 1990031 | cellular_component | pinceau fiber |
| A | 2000008 | biological_process | regulation of protein localization to cell surface |
| B | 0005216 | molecular_function | monoatomic ion channel activity |
| B | 0005249 | molecular_function | voltage-gated potassium channel activity |
| B | 0006811 | biological_process | monoatomic ion transport |
| B | 0006813 | biological_process | potassium ion transport |
| B | 0008076 | cellular_component | voltage-gated potassium channel complex |
| B | 0016020 | cellular_component | membrane |
| B | 0051260 | biological_process | protein homooligomerization |
| B | 0055085 | biological_process | transmembrane transport |
| C | 0002244 | biological_process | hematopoietic progenitor cell differentiation |
| C | 0004033 | molecular_function | aldo-keto reductase (NADPH) activity |
| C | 0005249 | molecular_function | voltage-gated potassium channel activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0005856 | cellular_component | cytoskeleton |
| C | 0005874 | cellular_component | microtubule |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0006813 | biological_process | potassium ion transport |
| C | 0008076 | cellular_component | voltage-gated potassium channel complex |
| C | 0009898 | cellular_component | cytoplasmic side of plasma membrane |
| C | 0014069 | cellular_component | postsynaptic density |
| C | 0015459 | molecular_function | potassium channel regulator activity |
| C | 0016020 | cellular_component | membrane |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0030424 | cellular_component | axon |
| C | 0034705 | cellular_component | potassium channel complex |
| C | 0043005 | cellular_component | neuron projection |
| C | 0043194 | cellular_component | axon initial segment |
| C | 0043679 | cellular_component | axon terminus |
| C | 0044224 | cellular_component | juxtaparanode region of axon |
| C | 0044325 | molecular_function | transmembrane transporter binding |
| C | 0044877 | molecular_function | protein-containing complex binding |
| C | 0045202 | cellular_component | synapse |
| C | 0045445 | biological_process | myoblast differentiation |
| C | 0050905 | biological_process | neuromuscular process |
| C | 0055085 | biological_process | transmembrane transport |
| C | 0055114 | biological_process | obsolete oxidation-reduction process |
| C | 0070995 | biological_process | NADPH oxidation |
| C | 0071805 | biological_process | potassium ion transmembrane transport |
| C | 0098839 | cellular_component | postsynaptic density membrane |
| C | 0098900 | biological_process | regulation of action potential |
| C | 0098978 | cellular_component | glutamatergic synapse |
| C | 1901379 | biological_process | regulation of potassium ion transmembrane transport |
| C | 1990002 | molecular_function | methylglyoxal reductase (NADPH) (acetol producing) activity |
| C | 1990031 | cellular_component | pinceau fiber |
| C | 2000008 | biological_process | regulation of protein localization to cell surface |
| D | 0005216 | molecular_function | monoatomic ion channel activity |
| D | 0005249 | molecular_function | voltage-gated potassium channel activity |
| D | 0006811 | biological_process | monoatomic ion transport |
| D | 0006813 | biological_process | potassium ion transport |
| D | 0008076 | cellular_component | voltage-gated potassium channel complex |
| D | 0016020 | cellular_component | membrane |
| D | 0051260 | biological_process | protein homooligomerization |
| D | 0055085 | biological_process | transmembrane transport |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE NAP A 1001 |
| Chain | Residue |
| A | GLY55 |
| A | ARG189 |
| A | GLN214 |
| A | TRP243 |
| A | SER244 |
| A | PRO245 |
| A | LEU246 |
| A | ALA247 |
| A | CYS248 |
| A | GLY249 |
| A | LYS254 |
| A | THR56 |
| A | SER263 |
| A | ARG264 |
| A | LEU321 |
| A | GLY323 |
| A | SER325 |
| A | GLN329 |
| A | GLU332 |
| A | ASN333 |
| A | HOH2036 |
| A | TRP57 |
| A | GLN63 |
| A | ASP85 |
| A | TYR90 |
| A | LYS118 |
| A | ASN158 |
| A | SER188 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PGW B 601 |
| Chain | Residue |
| B | ILE328 |
| B | PRO358 |
| B | PHE361 |
| B | TRP362 |
| B | LYS384 |
| B | SER388 |
| B | PGW605 |
| site_id | AC3 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE PGW B 602 |
| Chain | Residue |
| B | HOH2130 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PGW B 605 |
| Chain | Residue |
| B | VAL178 |
| B | PGW601 |
| site_id | AC5 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PGW B 606 |
| Chain | Residue |
| B | THR397 |
| B | PGW609 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PGW B 608 |
| Chain | Residue |
| B | SER307 |
| B | LYS308 |
| B | GLY309 |
| B | ARG322 |
| B | LEU326 |
| B | PHE329 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PGW B 609 |
| Chain | Residue |
| B | PHE330 |
| B | THR397 |
| B | PGW606 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PGW B 610 |
| Chain | Residue |
| B | ILE254 |
| B | MET255 |
| B | LYS308 |
| B | GLN311 |
| B | GLY314 |
| B | GLN315 |
| B | LYS318 |
| B | ARG415 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PGW B 611 |
| Chain | Residue |
| B | GLN214 |
| B | SER215 |
| B | THR216 |
| B | ILE231 |
| site_id | BC1 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE PGW B 612 |
| Chain | Residue |
| B | PHE218 |
| site_id | BC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE K B 501 |
| Chain | Residue |
| B | GLY372 |
| B | GLY372 |
| B | GLY372 |
| B | GLY372 |
| B | TYR373 |
| B | TYR373 |
| B | TYR373 |
| B | TYR373 |
| site_id | BC3 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE K B 502 |
| Chain | Residue |
| B | VAL371 |
| B | VAL371 |
| B | VAL371 |
| B | VAL371 |
| B | GLY372 |
| B | GLY372 |
| B | GLY372 |
| B | GLY372 |
| B | K503 |
| B | K503 |
| B | K503 |
| B | K503 |
| site_id | BC4 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE K B 503 |
| Chain | Residue |
| B | THR370 |
| B | THR370 |
| B | THR370 |
| B | THR370 |
| B | VAL371 |
| B | VAL371 |
| B | VAL371 |
| B | VAL371 |
| B | K502 |
| B | K502 |
| B | K502 |
| B | K502 |
| site_id | BC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE K B 504 |
| Chain | Residue |
| B | THR370 |
| B | THR370 |
| B | THR370 |
| B | THR370 |
| site_id | BC6 |
| Number of Residues | 30 |
| Details | BINDING SITE FOR RESIDUE NAP C 1001 |
| Chain | Residue |
| C | THR56 |
| C | TRP57 |
| C | GLN63 |
| C | ASP85 |
| C | TYR90 |
| C | ASN158 |
| C | SER188 |
| C | ARG189 |
| C | GLN214 |
| C | TRP243 |
| C | SER244 |
| C | PRO245 |
| C | LEU246 |
| C | ALA247 |
| C | CYS248 |
| C | GLY249 |
| C | SER252 |
| C | LYS254 |
| C | TYR262 |
| C | SER263 |
| C | ARG264 |
| C | LEU321 |
| C | GLY323 |
| C | SER325 |
| C | GLN329 |
| C | GLU332 |
| C | ASN333 |
| C | HOH2028 |
| C | HOH2038 |
| C | GLY55 |
| site_id | BC7 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PGW D 601 |
| Chain | Residue |
| D | PRO358 |
| D | PHE361 |
| D | TRP362 |
| D | VAL365 |
| D | ILE381 |
| D | LYS384 |
| D | SER388 |
| site_id | BC8 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE K D 501 |
| Chain | Residue |
| D | GLY372 |
| D | GLY372 |
| D | GLY372 |
| D | GLY372 |
| D | TYR373 |
| D | TYR373 |
| D | TYR373 |
| D | TYR373 |
| D | K502 |
| D | K502 |
| D | K502 |
| D | K502 |
| site_id | BC9 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE K D 502 |
| Chain | Residue |
| D | VAL371 |
| D | VAL371 |
| D | VAL371 |
| D | VAL371 |
| D | GLY372 |
| D | GLY372 |
| D | GLY372 |
| D | GLY372 |
| D | K501 |
| D | K501 |
| D | K501 |
| D | K501 |
| site_id | CC1 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE K D 503 |
| Chain | Residue |
| D | THR370 |
| D | THR370 |
| D | THR370 |
| D | THR370 |
| D | VAL371 |
| D | VAL371 |
| D | VAL371 |
| D | VAL371 |
| D | K504 |
| D | K504 |
| D | K504 |
| D | K504 |
| site_id | CC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE K D 504 |
| Chain | Residue |
| D | THR370 |
| D | THR370 |
| D | THR370 |
| D | THR370 |
| D | K503 |
| D | K503 |
| D | K503 |
| D | K503 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 366 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | MET1-GLY160 | |
| B | CYS244-ILE254 | |
| B | SER307-MET321 | |
| D | MET1-GLY160 | |
| D | CYS244-ILE254 | |
| D | SER307-MET321 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=Segment S1 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | PRO161-LEU182 | |
| A | LYS254 | |
| A | ARG264 | |
| A | SER325 | |
| A | GLN329 | |
| A | ASN333 | |
| C | THR56 | |
| C | TRP57 | |
| C | GLN63 | |
| C | ASP85 | |
| C | SER188 | |
| D | PRO161-LEU182 | |
| C | GLN214 | |
| C | TRP243 | |
| C | SER244 | |
| C | LEU246 | |
| C | LYS254 | |
| C | ARG264 | |
| C | SER325 | |
| C | GLN329 | |
| C | ASN333 | |
| A | GLN63 | |
| A | ASP85 | |
| A | SER188 | |
| A | GLN214 | |
| A | TRP243 | |
| A | SER244 | |
| A | LEU246 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 76 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430, ECO:0000305|PubMed:12151401 |
| Chain | Residue | Details |
| B | GLU183-PRO221 | |
| D | GLU183-PRO221 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=Segment S2 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | PHE222-ALA243 | |
| D | PHE222-ALA243 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 42 |
| Details | TRANSMEM: Helical; Name=Segment S5 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | ARG322-TYR343 | |
| D | ARG322-TYR343 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 38 |
| Details | TOPO_DOM: Extracellular => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | PHE344-ILE357 | |
| B | PRO378-LYS384 | |
| D | PHE344-ILE357 | |
| D | PRO378-LYS384 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 22 |
| Details | INTRAMEM: Helical; Name=Pore helix => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | PRO358-THR369 | |
| D | PRO358-THR369 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 14 |
| Details | INTRAMEM: INTRAMEM => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | THR370-VAL377 | |
| D | THR370-VAL377 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 56 |
| Details | TRANSMEM: Helical; Name=Segment S6 => ECO:0000269|PubMed:18004376, ECO:0000269|PubMed:20360102, ECO:0000269|PubMed:20534430 |
| Chain | Residue | Details |
| B | ILE385-TYR413 | |
| D | ILE385-TYR413 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 162 |
| Details | TOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:12151401, ECO:0000305 |
| Chain | Residue | Details |
| B | HIS414-VAL495 | |
| D | HIS414-VAL495 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | SITE: Important for normal, slow channel gating => ECO:0000269|PubMed:17766348 |
| Chain | Residue | Details |
| B | THR252 | |
| D | THR252 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | SITE: Important for binding with the scorpion mesomartoxin; when the scorpion mesomartoxin-rKv1.2/KCNA2 interaction is modeled, this residue is close to the 'Y-57' residue of the toxin => ECO:0000305|PubMed:25514171 |
| Chain | Residue | Details |
| B | VAL377 | |
| D | VAL377 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P63141 |
| Chain | Residue | Details |
| B | TYR425 | |
| D | TYR425 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P63141 |
| Chain | Residue | Details |
| B | SER430 | |
| D | SER430 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:18003609, ECO:0007744|PubMed:22673903 |
| Chain | Residue | Details |
| B | SER436 | |
| D | SER436 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:22673903 |
| Chain | Residue | Details |
| B | SER437 | |
| B | SER464 | |
| D | SER437 | |
| D | SER464 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:18003609, ECO:0000269|PubMed:21602278 |
| Chain | Residue | Details |
| B | SER445 | |
| D | SER445 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000305|PubMed:21602278 |
| Chain | Residue | Details |
| B | TYR454 | |
| D | TYR454 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 2 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000255 |
| Chain | Residue | Details |
| B | CYS244 | |
| D | CYS244 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255, ECO:0000269|PubMed:16770729 |
| Chain | Residue | Details |
| B | GLN207 | |
| D | GLN207 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 26 |
| Details | TOPO_DOM: Extracellular => ECO:0000250|UniProtKB:P63142 |
| Chain | Residue | Details |
| B | GLU274-ARG287 | |
| D | GLU274-ARG287 |
