3LMI

Crystal Structure of the Inactive Alpha-kinase Domain of Myosin Heavy Chain Kinase A (D766A) complex with ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Myosin heavy chain kinase A	polymer	307	34482.6	4	UniProt (P42527) Pfam (PF02816)	Dictyostelium discoideum (Slime mold)	MHCK-A
2	E, I, J, N, R... (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	6	Chemie (PO4)
3	F, K, O, T (A, B, C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
4	G, L, P, U (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	H, M, Q, V (A, B, C, D)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	4	Chemie (ATP)
6	W, X, Y, Z (A, B, C, D)	water	water		18.0	571	Chemie (HOH)

Sequence modifications

A, B, C, D: 552 - 841 (UniProt: P42527)

PDB	External Database	Details
Met 535	-	expression tag
Gly 536	-	expression tag
Gly 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
Gly 544	-	expression tag
Glu 545	-	expression tag
Asn 546	-	expression tag
Leu 547	-	expression tag
Tyr 548	-	expression tag
Phe 549	-	expression tag
Gln 550	-	expression tag
Gly 551	-	expression tag
Ala 766	Asp 766	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	137930.3
Non-Polymers*	Number of molecules	18
	Total formula weight	2957.4
All*	Total formula weight	140887.7

*Water molecules are not included.