3LKT

Tyrosine 447 of Protocatechuate 3,4-Dioxygenase Controls Efficient Progress Through Catalysis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Protocatechuate 3,4-dioxygenase alpha chain	polymer	200	22278.8	6	UniProt (P00436) Pfam (PF00775) In PDB	Pseudomonas putida	3,4-PCD
2	M, N, O, P, Q...	Protocatechuate 3,4-dioxygenase beta chain	polymer	238	26671.3	6	UniProt (P00437) Pfam (PF12391) Pfam (PF00775) In PDB	Pseudomonas putida	3,4-PCD
3	E, C, A, F, D...	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
4	P, N, Q, C, O...	BETA-MERCAPTOETHANOL	non-polymer		78.1	32	Chemie (BME)
5	B, E, F, A, D	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	5	Chemie (TRS)
6	N, Q, O, R, M...	FE (III) ION	non-polymer		55.8	6	Chemie (FE)
7	N, Q, O, R, M...	CHLORIDE ION	non-polymer		35.5	9	Chemie (CL)
8	C, M	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
9		water	water		18.0	2759	Chemie (HOH)

Sequence modifications

M, N, O, P, Q, R: 301 - 538 (UniProt: P00437)

PDB	External Database	Details
His 447	Tyr 148	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	293700.4
Non-Polymers*	Number of molecules	60
	Total formula weight	4525.7
All*	Total formula weight	298226.1

*Water molecules are not included.