3L4Q

Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Non-structural protein 1	polymer	164	18464.2	2	UniProt (P03496) Pfam (PF00600)	Influenza A virus	NS1, NS1A
2	B, D (C, D)	Phosphatidylinositol 3-kinase regulatory subunit beta	polymer	170	20625.2	2	UniProt (P23726) Pfam (PF16454)	Bos taurus (bovine)	P85BETA, PI3-kinase p85 subunit beta, PtdIns-3-kinase p85-beta
3	E, F (C, D)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	G, H, I, J (A, C, B, D)	water	water		18.0	281	Chemie (HOH)

Sequence modifications

A, B: 73 - 230 (UniProt: P03496)

PDB	External Database	Details
His 67	-	expression tag
His 68	-	expression tag
His 69	-	expression tag
His 70	-	expression tag
His 71	-	expression tag
His 72	-	expression tag

C, D: 422 - 591 (UniProt: P23726)

PDB	External Database	Details
Ser 495	Cys 497	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	78178.8
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	78363.0

*Water molecules are not included.