3L02

Crystal structure of N-acetyl-L-ornithine transcarbamylase E92A mutant complexed with carbamyl phosphate and N-succinyl-L-norvaline

Replaces:  2G65

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	N-acetylornithine carbamoyltransferase	polymer	359	40075.5	1	UniProt (Q8P8J2) Pfam (PF02729) Pfam (PF00185)	Xanthomonas campestris pv. campestris	AOTCase
2	B (A)	N-(3-CARBOXYPROPANOYL)-L-NORVALINE	non-polymer		217.2	1	Chemie (SN0)
3	C (A)	PHOSPHORIC ACID MONO(FORMAMIDE)ESTER	non-polymer		141.0	1	Chemie (CP)
4	D, E (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	F (A)	water	water		18.0	108	Chemie (HOH)

Sequence modifications

A: 1 - 339 (UniProt: Q8P8J2)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 92	Glu 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40075.5
Non-Polymers*	Number of molecules	4
	Total formula weight	550.4
All*	Total formula weight	40625.9

*Water molecules are not included.