3KO2

I-MsoI re-designed for altered DNA cleavage specificity (-7C)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, F, G)	Site-specific DNA endonuclease I-MsoI	polymer	170	19601.5	4	UniProt (C0JWR6) Pfam (PF00961)	Monomastix sp.
2	C, G (C, H)	5'-D(*GP*CP*AP*GP*AP*CP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3'	polymer	24	7395.8	2
3	D, H (D, I)	5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*GP*TP*CP*TP*GP*C)-3'	polymer	24	7346.7	2
4	I, J, K, L, M... (A, C, F, H)	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
5	O, P, Q, R (A, B, F, G)	water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, B, F, G: 1 - 170 (UniProt: C0JWR6)

PDB	External Database	Details
Arg 28	Lys 28	engineered mutation
Glu 43	Ser 43	engineered mutation
Thr 70	Asn 70	engineered mutation
Trp 85	Ile 85	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	107891.1
Non-Polymers*	Number of molecules	6
	Total formula weight	240.5
All*	Total formula weight	108131.5

*Water molecules are not included.