3KO2
I-MsoI re-designed for altered DNA cleavage specificity (-7C)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, F, G | Site-specific DNA endonuclease I-MsoI | polymer | 170 | 19601.5 | 4 | UniProt (C0JWR6) Pfam (PF00961) In PDB | Monomastix sp. | |
2 | C, H | 5'-D(*GP*CP*AP*GP*AP*CP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3' | polymer | 24 | 7395.8 | 2 | |||
3 | D, I | 5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*GP*TP*CP*TP*GP*C)-3' | polymer | 24 | 7346.7 | 2 | |||
4 | A, C, F, H | CALCIUM ION | non-polymer | 40.1 | 6 | Chemie (CA) | |||
5 | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A, B, F, G: 1 - 170 (UniProt: C0JWR6)
PDB | External Database | Details |
---|---|---|
Arg 28 | Lys 28 | engineered mutation |
Glu 43 | Ser 43 | engineered mutation |
Thr 70 | Asn 70 | engineered mutation |
Trp 85 | Ile 85 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 107891.1 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 240.5 | |
All* | Total formula weight | 108131.5 |