3KLF
Crystal structure of wild-type HIV-1 Reverse Transcriptase crosslinked to a DSDNA with a bound excision product, AZTPPPPA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (A, E, I, M) | Reverse transcriptase/ribonuclease H | polymer | 557 | 64080.5 | 4 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) | Human immunodeficiency virus type 1 (HIV-1) | p66 RT |
| 2 | B, D, F, H (B, F, J, N) | p51 RT | polymer | 444 | 51928.6 | 4 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) | Human immunodeficiency virus type 1 (HIV-1) | |
| 3 | I, K, M, O (C, G, K, O) | DNA (5'-D(*AP*T*GP*CP*AP*TP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*GP*TP*G)-3') | polymer | 27 | 8367.4 | 4 | |||
| 4 | J, L, N, P (D, H, L, P) | DNA (5'-D(*A*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*(MRG)P*CP*GP*CP*CP*(2DA))-3') | polymer | 21 | 6458.3 | 4 | |||
| 5 | BA, CA, Q, R, U... (M, A, E, I) | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) | |||
| 6 | AA, DA, S, W (I, M, A, E) | [[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl] [(2S,3S,5R)-3-azido-5-(5-methyl-2,4-dioxo-pyrimidin-1-yl)oxolan-2-yl]methyl hydrogen phosphate | non-polymer | 836.4 | 4 | Chemie (ZP4) | |||
| 7 | T, X (B, F) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 8 | EA, FA, GA, HA, IA... (A, B, E, F, I...) | water | water | 18.0 | 76 | Chemie (HOH) |
Sequence modifications
A, E, I, M: 1 - 555 (UniProt: P03366)
B, F, J, N: 1 - 428 (UniProt: P03366)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Val 0 | - | expression tag |
| Cys 258 | Gln 857 | engineered mutation |
| Ser 280 | Cys 879 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | expression tag |
| Ala -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Val -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ser 280 | Cys 879 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 523339.0 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 3724.2 | |
| All* | Total formula weight | 527063.1 |
