3KHL

Dpo4 post-extension ternary complex with misinserted A opposite the 2-aminofluorene-guanine [AF]G lesion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA polymerase IV	polymer	341	38945.4	2	UniProt (Q97W02) Pfam (PF00817) Pfam (PF11798) Pfam (PF11799) In PDB	Sulfolobus solfataricus P2	Pol IV
2	D, H	5'-D(*TP*TP*GP*GP*AP*TP*GP*GP*TP*AP*GP*AP*(DDG))-3'	polymer	13	4070.7	2
3	E, J	5'-D(*CP*C*TP*AP*AP*CP*GP*CP*TP*AP*CP*CP*AP*TP*CP*CP*AP*AP*CP*C)-3'	polymer	20	5967.9	2
4	A, B	THYMIDINE-5'-TRIPHOSPHATE	non-polymer		482.2	2	Chemie (TTP)
5	A, B	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
6	E, J	2-AMINOFLUORENE	non-polymer		181.2	2	Chemie (AF)
7		water	water		18.0	316	Chemie (HOH)

Sequence modifications

A, B: 2 - 341 (UniProt: Q97W02)

PDB	External Database	Details
Gly 1	-	expression tag

D, H: 803 - 815 (PDB: 3KHL)

PDB	External Database	Details
Ddg 815	G 13	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	97967.9
Non-Polymers*	Number of molecules	10
	Total formula weight	1567.3
All*	Total formula weight	99535.1

*Water molecules are not included.