3KD5

Closed ternary complex of an RB69 gp43 fingers domain mutant complexed with an acyclic GMP terminated primer template pair and phosphonoformic acid.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (T)	DNA (5'-D(*CP*GP*TP*CP*TP*TP*AP*TP*GP*AP*CP*AP*GP*CP*CP*GP*CP*G)-3')	polymer	18	5492.6	1
2	B (P)	DNA (5'-D(*GP*CP*GP*GP*CP*TP*GP*TP*CP*AP*TP*AP*AP*(4DG))-3')	polymer	14	4278.8	1
3	C (E)	DNA polymerase	polymer	913	105806.4	1	UniProt (Q38087) Pfam (PF03104) Pfam (PF00136)	Enterobacteria phage RB69 (Bacteriophage RB69)	Gp43
4	D (E)	PHOSPHONOFORMIC ACID	non-polymer		126.0	1	Chemie (PPF)
5	E, F, G (E)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
6	H, I, J (T, P, E)	water	water		18.0	246	Chemie (HOH)

Sequence modifications

E: 1 - 903 (UniProt: Q38087)

PDB	External Database	Details
Ala 222	Asp 222	engineered mutation
Trp 478	Val 478	engineered mutation
Val 479	Phe 479	engineered mutation
Ser 480	Asn 480	engineered mutation
Met 557	Ile 557	engineered mutation
Ala 558	Asn 558	engineered mutation
Leu 559	Arg 559	engineered mutation
Val 561	Leu 561	engineered mutation
Thr 562	Leu 562	engineered mutation
Cys 563	Ile 563	engineered mutation
Ser 904	-	expression tag
Ala 905	-	expression tag
Trp 906	-	expression tag
Ser 907	-	expression tag
His 908	-	expression tag
Pro 909	-	expression tag
Gln 910	-	expression tag
Phe 911	-	expression tag
Glu 912	-	expression tag
Lys 913	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	115577.7
Non-Polymers*	Number of molecules	4
	Total formula weight	198.9
All*	Total formula weight	115776.6

*Water molecules are not included.