3KD1
Closed binary complex of an RB69 gp43 fingers domain mutant complexed with an acyclic GMP terminated primer template pair.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (T) | DNA (5'-D(*CP*GP*TP*CP*TP*TP*AP*TP*GP*AP*CP*AP*GP*CP*CP*GP*CP*G)-3') | polymer | 18 | 5492.6 | 1 | |||
| 2 | B (P) | DNA (5'-D(*GP*CP*GP*GP*CP*TP*GP*TP*CP*AP*TP*AP*AP*(4DG))-3') | polymer | 14 | 4278.8 | 1 | |||
| 3 | C (E) | DNA polymerase | polymer | 913 | 105806.4 | 1 | UniProt (Q38087) Pfam (PF03104) Pfam (PF00136) | Enterobacteria phage RB69 (Bacteriophage RB69) | Gp43 |
| 4 | D (E) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E, F, G (T, P, E) | water | water | 18.0 | 321 | Chemie (HOH) |
Sequence modifications
E: 1 - 903 (UniProt: Q38087)
| PDB | External Database | Details |
|---|---|---|
| Ala 222 | Asp 222 | engineered mutation |
| Trp 478 | Val 478 | engineered mutation |
| Val 479 | Phe 479 | engineered mutation |
| Ser 480 | Asn 480 | engineered mutation |
| Met 557 | Ile 557 | engineered mutation |
| Ala 558 | Asn 558 | engineered mutation |
| Leu 559 | Arg 559 | engineered mutation |
| Val 561 | Leu 561 | engineered mutation |
| Thr 562 | Leu 562 | engineered mutation |
| Cys 563 | Ile 563 | engineered mutation |
| Ser 904 | - | expression tag |
| Ala 905 | - | expression tag |
| Trp 906 | - | expression tag |
| Ser 907 | - | expression tag |
| His 908 | - | expression tag |
| Pro 909 | - | expression tag |
| Gln 910 | - | expression tag |
| Phe 911 | - | expression tag |
| Glu 912 | - | expression tag |
| Lys 913 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 115577.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 115602.0 |
