3KD1
Closed binary complex of an RB69 gp43 fingers domain mutant complexed with an acyclic GMP terminated primer template pair.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-15 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.03315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.485, 121.657, 131.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.660 |
| R-factor | 0.2119 |
| Rwork | 0.208 |
| R-free | 0.24826 |
| Structure solution method | isomorphous replacement |
| Starting model (for MR) | 1ih7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.916 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.760 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.106 | |
| Number of reflections | 36963 | |
| <I/σ(I)> | 17.7 | 2.24 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 5% PEG 20000, 0.1M sodium acetate pH 5, 0.1M magnesium acetate, 0.1M Tris-HCl pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
