3K5M
Crystal structure of E.coli Pol II-abasic DNA-ddGTP Lt(-2, 2) ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase II | polymer | 786 | 90459.7 | 1 | UniProt (P21189) Pfam (PF21474) Pfam (PF22587) Pfam (PF03104) Pfam (PF00136) | Escherichia coli | Pol II |
| 2 | B (T) | DNA (5'-D(*AP*GP*TP*CP*CP*TP*GP*(3DR)P*AP*CP*GP*CP*TP*AP*GP*GP*CP*AP*CP*A)-3') | polymer | 20 | 5994.9 | 1 | |||
| 3 | C (P) | DNA (5'-D(*GP*TP*GP*CP*CP*TP*AP*GP*CP*GP*TP*AP*G)-3') | polymer | 13 | 4007.6 | 1 | |||
| 4 | D (A) | 2'-3'-DIDEOXYGUANOSINE-5'-TRIPHOSPHATE | non-polymer | 491.2 | 1 | Chemie (DG3) | |||
| 5 | E, F (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | G, H, I (A, T, P) | water | water | 18.0 | 491 | Chemie (HOH) |
Sequence modifications
A: 1 - 783 (UniProt: P21189)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| Asn 335 | Asp 335 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 100462.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 571.3 | |
| All* | Total formula weight | 101033.6 |
