3K0C

Crystal structure of the phosphorylation-site double mutant S431A/T432E of the KaiC circadian clock protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, E, F)	Circadian clock protein kinase KaiC	polymer	519	58164.8	4	UniProt (Q79PF4) Pfam (PF06745)	Synechococcus elongatus PCC 7942 (Anacystis nidulans R2)
2	C, D (C, D)	Circadian clock protein kinase KaiC	polymer	519	58084.8	2	UniProt (Q79PF4) Pfam (PF06745)	Synechococcus elongatus PCC 7942 (Anacystis nidulans R2)
3	FA, G, GA, H, JA... (E, A, F, B, C...)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	12	Chemie (ATP)
4	AA, BA, CA, DA, EA... (D, E, A, F, B...)	MAGNESIUM ION	non-polymer		24.3	22	Chemie (MG)
5	OA, PA, QA, RA, SA... (A, B, C, D, E...)	water	water		18.0	117	Chemie (HOH)

Sequence modifications

A, B, E, F: 1 - 519 (UniProt: Q79PF4)

PDB	External Database	Details
Ala 431	Ser 431	engineered mutation
Glu 432	Thr 432	engineered mutation

C, D: 1 - 519 (UniProt: Q79PF4)

PDB	External Database	Details
Ala 431	Ser 431	engineered mutation
Glu 432	Thr 432	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	348828.6
Non-Polymers*	Number of molecules	34
	Total formula weight	6620.9
All*	Total formula weight	355449.5

*Water molecules are not included.