3K0C

Crystal structure of the phosphorylation-site double mutant S431A/T432E of the KaiC circadian clock protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	113.15
Detector technology	CCD
Collection date	2008-10-23
Detector	MAR 300MM CCD
Spacegroup name	P 21 21 21
Unit cell lengths	132.283, 135.031, 204.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.300
R-factor	0.231
Rwork	0.231
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gbl
Data reduction software	HKL-2000
Data scaling software	XSCALE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.400
High resolution limit [Å]	3.300	3.300
Rmerge	0.103	0.574
Number of reflections	54512
<I/σ(I)>	13.8	2.3
Completeness [%]	98.3	88.7
Redundancy	6.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4	291	SODIUM FORMATE, GLYCEROL, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 291K