3JVZ

E2~Ubiquitin-HECT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ubiquitin-conjugating enzyme E2 D2	polymer	146	16610.0	2	UniProt (P62837) Pfam (PF00179) In PDB	Homo sapiens (human)	Ubiquitin-protein ligase D2, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2-17 kDa 2, E2(17)KB 2
2	C, D	E3 ubiquitin-protein ligase NEDD4-like	polymer	385	45435.6	2	UniProt (Q96PU5) Pfam (PF00632) In PDB	Homo sapiens (human)	Nedd4-2, NEDD4.2
3	X, Y	Ubiquitin	polymer	81	8922.1	2	UniProt (P62988) Pfam (PF00240) UniProt (by SIFTS) (P0CG48) In PDB	Homo sapiens

Sequence modifications

A, B: 2 - 147 (UniProt: P62837)

PDB	External Database	Details
Ser 3	Leu 3	engineered mutation
Ser 85	Cys 85	engineered mutation
Lys 98	Thr 98	engineered mutation

C, D: 576 - 955 (UniProt: Q96PU5)

PDB	External Database	Details
Gly 571	-	expression tag
Ser 572	-	expression tag
Pro 573	-	expression tag
Glu 574	-	expression tag
Phe 575	-	expression tag
Ala 922	Cys 942	engineered mutation

X, Y: 1 - 76 (UniProt: P62988)

PDB	External Database	Details
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	141935.5
All*	Total formula weight	141935.5

*Water molecules are not included.