3JCR

3D structure determination of the human*U4/U6.U5* tri-snRNP complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (G)	hPrp6	polymer	941	107092.2	1	UniProt (O94906) Pfam (PF06424) Pfam (PF14559) Pfam (PF13432)	Homo sapiens (human)	Pre-mRNA-processing factor 6, Androgen receptor N-terminal domain-transactivating protein 1, ANT-1, PRP6 homolog, U5 snRNP-associated 102 kDa protein, U5-102 kDa protein
2	B (D)	U5-40K	polymer	357	39359.5	1	UniProt (Q96DI7) Pfam (PF00400)	Homo sapiens (human)	U5 small nuclear ribonucleoprotein 40 kDa protein, U5 snRNP 40 kDa protein, U5-40K, 38 kDa-splicing factor, Prp8-binding protein, hPRP8BP, U5 snRNP-specific 40 kDa protein, WD repeat-containing protein 57
3	C (C)	hBrr2	polymer	2136	244823.4	1	UniProt (O75643) Pfam (PF21188) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF02889)	Homo sapiens (human)	U5 small nuclear ribonucleoprotein 200 kDa helicase, Activating signal cointegrator 1 complex subunit 3-like 1, BRR2 homolog, U5 snRNP-specific 200 kDa protein, U5-200KD
4	D (E)	hDim1	polymer	142	16807.3	1	UniProt (P83876) Pfam (PF02966)	Homo sapiens (human)	Thioredoxin-like protein 4A, DIM1 protein homolog, Spliceosomal U5 snRNP-specific 15 kDa protein, Thioredoxin-like U5 snRNP protein U5-15kD
5	E (A)	hPrp8	polymer	2335	273974.3	1	UniProt (Q6P2Q9) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF01398) Pfam (PF08084) Pfam (PF08082) Pfam (PF08083)	Homo sapiens (human)	Pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220
6	F (F)	hPrp28	polymer	820	95785.1	1	UniProt (Q9BUQ8) Pfam (PF00270) Pfam (PF00271)	Homo sapiens (human)	Probable ATP-dependent RNA helicase DDX23, 100 kDa U5 snRNP-specific protein, DEAD box protein 23, PRP28 homolog, U5-100kD
7	G (B)	hSnu114	polymer	972	109560.6	1	UniProt (Q15029) Pfam (PF16004) Pfam (PF00009) Pfam (PF03144) Pfam (PF14492) Pfam (PF03764) Pfam (PF00679)	Homo sapiens (human)	116 kDa U5 small nuclear ribonucleoprotein component, Elongation factor Tu GTP-binding domain-containing protein 2, SNU114 homolog, hSNU114, U5 snRNP-specific protein, 116 kDa, U5-116 kDa
8	H, Z (O, o)	SmB	polymer	240	24642.1	2	UniProt (P14678) Pfam (PF01423)	Homo sapiens (human)	Small nuclear ribonucleoprotein-associated proteins B and B', snRNP-B, Sm protein B/B', Sm-B/B', SmB/B'
9	AA, I (P, p)	SmD1	polymer	119	13310.7	2	UniProt (P62314) Pfam (PF01423)	Homo sapiens (human)	Small nuclear ribonucleoprotein Sm D1, Sm-D1, Sm-D autoantigen, snRNP core protein D1
10	BA, J (Q, q)	SmD2	polymer	118	13551.9	2	UniProt (P62316) Pfam (PF01423)	Homo sapiens (human)	Small nuclear ribonucleoprotein Sm D2, Sm-D2, snRNP core protein D2
11	CA, K (R, r)	SmD3	polymer	126	13940.3	2	UniProt (P62318) Pfam (PF01423)	Homo sapiens (human)	Small nuclear ribonucleoprotein Sm D3, Sm-D3, snRNP core protein D3
12	DA, L (S, s)	SmE	polymer	92	10817.6	2	UniProt (P62304) Pfam (PF01423)	Homo sapiens (human)	Small nuclear ribonucleoprotein E, snRNP-E, Sm protein E, Sm-E, SmE
13	EA, M (T, t)	SmF	polymer	86	9734.2	2	UniProt (P62306) Pfam (PF01423)	Homo sapiens (human)	Small nuclear ribonucleoprotein F, snRNP-F, Sm protein F, Sm-F, SmF
14	FA, N (U, u)	SmG	polymer	76	8508.1	2	UniProt (P62308) Pfam (PF01423)	Homo sapiens (human)	Small nuclear ribonucleoprotein G, snRNP-G, Sm protein G, Sm-G, SmG
15	O (8)	LSm8	polymer	96	10410.6	1	UniProt (O95777) Pfam (PF01423)	Homo sapiens (human)	U6 snRNA-associated Sm-like protein LSm8
16	P (6)	LSm6	polymer	80	9139.6	1	UniProt (P62312) Pfam (PF01423)	Homo sapiens (human)	U6 snRNA-associated Sm-like protein LSm6
17	Q (5)	LSm5	polymer	91	9945.4	1	UniProt (Q9Y4Y9) Pfam (PF01423)	Homo sapiens (human)	U6 snRNA-associated Sm-like protein LSm5
18	R (4)	LSm4	polymer	139	15375.8	1	UniProt (Q9Y4Z0) Pfam (PF01423)	Homo sapiens (human)	U6 snRNA-associated Sm-like protein LSm4, Glycine-rich protein, GRP
19	S (3)	LSm3	polymer	102	11859.4	1	UniProt (P62310) Pfam (PF01423)	Homo sapiens (human)	U6 snRNA-associated Sm-like protein LSm3
20	T (2)	LSm2	polymer	95	10847.5	1	UniProt (Q9Y333) Pfam (PF01423)	Homo sapiens (human)	U6 snRNA-associated Sm-like protein LSm2, Protein G7b, Small nuclear ribonuclear protein D homolog, snRNP core Sm-like protein Sm-x5
21	U (7)	LSm7	polymer	103	11617.4	1	UniProt (Q9UK45) Pfam (PF01423)	Homo sapiens (human)	U6 snRNA-associated Sm-like protein LSm7
22	V (K)	hPrp3	polymer	683	77669.2	1	UniProt (O43395) Pfam (PF01480) Pfam (PF08572) Pfam (PF06544)	Homo sapiens (human)	U4/U6 small nuclear ribonucleoprotein Prp3, Pre-mRNA-splicing factor 3, hPrp3, U4/U6 snRNP 90 kDa protein
23	W (L)	hPrp4	polymer	521	58408.0	1	UniProt (Q5T1M7) Pfam (PF08799) Pfam (PF00400) UniProt (by SIFTS) (O43172)	Homo sapiens (human)	60 kDa U4/U6 snRNP-specific spliceosomal protein, PRP4 pre-mRNA processing factor 4 homolog (Yeast), isoform CRA_b
24	X (J)	hPrp31	polymer	499	55529.0	1	UniProt (Q8WWY3) Pfam (PF01798) Pfam (PF09785)	Homo sapiens (human)	U4/U6 small nuclear ribonucleoprotein Prp31, Pre-mRNA-processing factor 31, Serologically defined breast cancer antigen NY-BR-99, U4/U6 snRNP 61 kDa protein, Protein 61K, hPrp31
25	Y (I)	hSnu13	polymer	128	14191.5	1	UniProt (P55769) Pfam (PF01248)	Homo sapiens (human)	NHP2-like protein 1, High mobility group-like nuclear protein 2 homolog 1, OTK27, SNU13 homolog, hSNU13, U4/U6.U5 small nuclear ribonucleoprotein SNU13, U4/U6.U5 tri-snRNP 15.5 kDa protein, NHP2-like protein 1, N-terminally processed
26	GA (V)	hSad1	polymer	565	65481.4	1	UniProt (Q53GS9) Pfam (PF02148) Pfam (PF00443)	Homo sapiens (human)	U4/U6.U5 tri-snRNP-associated protein 2, Inactive ubiquitin-specific peptidase 39, SAD1 homolog, U4/U6.U5 tri-snRNP-associated 65 kDa protein, 65K
27	HA (M)	U4 snRNA	polymer	145	46536.5	1		Homo sapiens (human)
28	IA (N)	U6 snRNA	polymer	106	34098.3	1		Homo sapiens (human)
29	JA (H)	U5 snRNA	polymer	116	36891.7	1		Homo sapiens (human)

Contents of the asymmetric unit

Polymers	Number of chains	36
	Total formula weight	1544413.4
All*	Total formula weight	1544413.4

*Water molecules are not included.