3JBW

Cryo-electron microscopy structure of RAG Paired Complex (with NBD, no symmetry)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	V(D)J recombination-activating protein 1	polymer	764	87684.1	2	UniProt (O13033) Pfam (PF00097) Pfam (PF12940)	Danio rerio (zebra fish)	RAG-1, Endonuclease RAG1, E3 ubiquitin-protein ligase RAG1
2	B, D (B, D)	V(D)J recombination-activating protein 2	polymer	533	59435.9	2	UniProt (Q1RLW7) Pfam (PF03089) Pfam (PF13341) UniProt (by SIFTS) (O13034)	Danio rerio (zebra fish)	RAG-2
3	E (E)	12-RSS signal end forward strand	polymer	34	10439.8	1		Homo sapiens
4	F (F)	Nicked 12-RSS intermediate reverse strand	polymer	50	15439.9	1		Homo sapiens
5	G (G)	Nicked 23-RSS intermediate reverse strand	polymer	61	18870.1	1		Homo sapiens
6	H (H)	Nicked 23-RSS intermediate forward strand	polymer	45	13806.9	1		Homo sapiens
7	I, J (I, J)	5'-D(P*GP*AP*TP*CP*TP*GP*GP*CP*CP*TP*GP*TP*CP*TP*TP*A)-3'	polymer	16	4880.2	2		Homo sapiens
8	K, L (A, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, C: 271 - 1031 (UniProt: O13033)

PDB	External Database	Details
Gly 268	-	expression tag
Gly 269	-	expression tag
Ser 270	-	expression tag

B, D: 1 - 530 (UniProt: Q1RLW7)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	362557.0
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	362687.9

*Water molecules are not included.