3JBW
Cryo-electron microscopy structure of RAG Paired Complex (with NBD, no symmetry)
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
| Method | Blot for 2.5 seconds before plunging. |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 550 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.6 |
| Software | RELION,SPIDER |
| Symmetry | C1 |
