3JAP

Structure of a partial yeast 48S preinitiation complex in closed conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	1	Met-tRNAi (U31:A39 variant)	polymer	75	24223.5	1		Kluyveromyces lactis
2	2	18S rRNA	polymer	1781	573814.1	1		Kluyveromyces lactis
3	3	mRNA	polymer	25	7786.6	1		synthetic construct
4	A	uS2	polymer	254	28264.5	1	UniProt (Q6CN12) Pfam (PF00318) In PDB	Kluyveromyces lactis
5	B	eS1	polymer	255	28971.6	1	UniProt (Q6CWD0) Pfam (PF01015) In PDB	Kluyveromyces lactis
6	C	uS5	polymer	259	27650.0	1	UniProt (Q6CKL3) Pfam (PF00333) Pfam (PF03719) In PDB	Kluyveromyces lactis
7	D	uS3	polymer	237	26300.5	1	UniProt (Q6CRK7) Pfam (PF07650) Pfam (PF00189) In PDB	Kluyveromyces lactis
8	E	eS4	polymer	261	29617.5	1	UniProt (Q6CWJ2) Pfam (PF00900) Pfam (PF00467) Pfam (PF16121) Pfam (PF08071) Pfam (PF01479) In PDB	Kluyveromyces lactis
9	F	uS7	polymer	227	25386.0	1	UniProt (Q6CRA3) Pfam (PF00177) In PDB	Kluyveromyces lactis
10	G	eS6	polymer	236	26970.4	1	UniProt (Q6CM04) Pfam (PF01092) In PDB	Kluyveromyces lactis
11	H	eS7	polymer	190	21735.3	1	UniProt (Q6CTD6) Pfam (PF01251) In PDB	Kluyveromyces lactis
12	I	eS8	polymer	201	22642.7	1	UniProt (Q6CMG3) Pfam (PF01201) In PDB	Kluyveromyces lactis
13	J	uS4	polymer	188	21587.0	1	UniProt (Q6CM18) Pfam (PF00163) Pfam (PF01479) In PDB	Kluyveromyces lactis
14	K	eS10	polymer	106	12584.4	1	UniProt (Q6CVZ5) Pfam (PF03501) In PDB	Kluyveromyces lactis
15	L	uS17	polymer	156	17843.9	1	UniProt (Q6CX80) Pfam (PF16205) Pfam (PF00366) In PDB	Kluyveromyces lactis
16	M	eS12	polymer	134	14466.4	1	UniProt (Q6CLU4) Pfam (PF01248) In PDB	Kluyveromyces lactis
17	N	uS15	polymer	151	16989.9	1	UniProt (Q6CJK0) Pfam (PF08069) Pfam (PF00312) In PDB	Kluyveromyces lactis
18	O	uS11	polymer	137	14530.7	1	UniProt (P27069) Pfam (PF00411) In PDB	Kluyveromyces lactis
19	P	uS19	polymer	142	15986.8	1	UniProt (Q6CKV4) Pfam (PF00203) In PDB	Kluyveromyces lactis
20	Q	uS9	polymer	143	15874.5	1	UniProt (Q875N2) Pfam (PF00380) In PDB	Kluyveromyces lactis
21	R	eS17	polymer	136	15722.2	1	UniProt (Q6CWU3) Pfam (PF00833) In PDB	Kluyveromyces lactis
22	S	uS13	polymer	146	17084.6	1	UniProt (Q6CWT9) Pfam (PF00416) In PDB	Kluyveromyces lactis
23	T	eS19	polymer	144	15879.0	1	UniProt (Q6CXM0) Pfam (PF01090) In PDB	Kluyveromyces lactis
24	U	uS10	polymer	117	13337.6	1	UniProt (Q6CIM1) Pfam (PF00338) In PDB	Kluyveromyces lactis
25	V	eS21	polymer	87	9797.9	1	UniProt (Q6CXT6) Pfam (PF01249) In PDB	Kluyveromyces lactis
26	W	uS8	polymer	130	14645.0	1	UniProt (Q6CW21) Pfam (PF00410) In PDB	Kluyveromyces lactis
27	X	uS12	polymer	145	16047.9	1	UniProt (F2Z602) Pfam (PF00164) In PDB	Kluyveromyces lactis
28	Y	eS24	polymer	135	15194.5	1	UniProt (Q6CU44) Pfam (PF01282) In PDB	Kluyveromyces lactis
29	Z	eS25	polymer	108	12002.1	1	UniProt (Q6CW78) Pfam (PF03297) In PDB	Kluyveromyces lactis
30	a	eS26	polymer	119	13540.0	1	UniProt (Q6CS01) Pfam (PF01283) In PDB	Kluyveromyces lactis
31	b	eS27	polymer	82	8884.4	1	UniProt (Q6CNL2) Pfam (PF01667) In PDB	Kluyveromyces lactis
32	c	eS28	polymer	67	7549.8	1	UniProt (P33285) Pfam (PF01200) In PDB	Kluyveromyces lactis
33	d	uS14	polymer	56	6662.6	1	UniProt (Q6CPG3) Pfam (PF00253) In PDB	Kluyveromyces lactis
34	e	eS30	polymer	63	7141.4	1	UniProt (Q6CUH5) Pfam (PF04758) In PDB	Kluyveromyces lactis
35	f	eS31	polymer	150	17111.0	1	UniProt (P69061) Pfam (PF00240) Pfam (PF01599) In PDB	Kluyveromyces lactis
36	g	RACK1	polymer	326	35830.9	1	UniProt (Q6CNI7) Pfam (PF00400) In PDB	Kluyveromyces lactis
37	h	eL41	polymer	25	3354.2	1	UniProt (P0CX86) Pfam (PF05162) In PDB	Kluyveromyces lactis
38	i	eIF1A	polymer	153	17462.2	1	UniProt (P38912) Pfam (PF01176) In PDB	Saccharomyces cerevisiae (yeast)
39	j	eIF2 alpha	polymer	304	34763.7	1	UniProt (P20459) Pfam (PF00575) Pfam (PF07541) In PDB	Saccharomyces cerevisiae (yeast)
40	k	eIF2 gamma	polymer	527	57942.7	1	UniProt (P32481) Pfam (PF00009) Pfam (PF03144) Pfam (PF09173) In PDB	Saccharomyces cerevisiae (yeast)
41	l	eIF2 beta	polymer	285	31631.3	1	UniProt (P09064) Pfam (PF01873) In PDB	Saccharomyces cerevisiae (yeast)
42	m	eIF1	polymer	108	12330.1	1	UniProt (P32911) Pfam (PF01253) In PDB	Saccharomyces cerevisiae (yeast)
43	o	eIF3a	polymer	588	64752.1	1	UniProt (P38249) In PDB	Saccharomyces cerevisiae (yeast)
44	p	eIF3c	polymer	652	73397.4	1	UniProt (P32497) Pfam (PF05470) Pfam (PF01399) In PDB	Saccharomyces cerevisiae (yeast)
45	q	eIF3i	polymer	347	38803.4	1	UniProt (P40217) Pfam (PF00400) In PDB	Saccharomyces cerevisiae (yeast)
46	r	eIF3b	polymer	31	3963.6	1	UniProt (P06103) In PDB	Saccharomyces cerevisiae (yeast)
47	s	eIF3g	polymer	52	5942.6	1	UniProt (A6ZZ25) Pfam (PF12353) In PDB	Saccharomyces cerevisiae (yeast)
48	2, k	MAGNESIUM ION	non-polymer		24.3	81	Chemie (MG)
49	l, a, b, f	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
50	k	METHIONINE	non-polymer		149.2	1	Chemie (MET)
51	k	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	non-polymer		521.2	1	Chemie (GCP)

Sequence modifications

o: 1 - 495 (UniProt: P38249)
p: 193 - 206 (UniProt: P32497)
p: 251 - 812 (UniProt: P32497)
o: 1 - 866 (PDB: 3JAP)
p: 20 - 812 (PDB: 3JAP)

Contents of the asymmetric unit

Polymers	Number of chains	47
	Total formula weight	1544000.6
Non-Polymers*	Number of molecules	87
	Total formula weight	2900.8
All*	Total formula weight	1546901.4

*Water molecules are not included.