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3JAP

Structure of a partial yeast 48S preinitiation complex in closed conformation

EM specimen
VitrificationYes
InstrumentFEI VITROBOT MARK I
MethodBlot for 2.5 to 3 seconds before plunging
EM Data Collection
MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)2.7
DetectorFEI FALCON II (4k x 4k)
Number of Images5500
3D Reconstruction
MethodSINGLE PARTICLE
Resolution (Å)4.9
SoftwareCoot,REFMAC,UCSF Chimera,RELION
SymmetryC1

217705

PDB entries from 2024-03-27

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