3JAK
Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 (merged dataset with and without kinesin bound)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D, F, H, J... (E, J, C, L, A...) | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 6 | UniProt (Q2XVP4) Pfam (PF00091) Pfam (PF03953) | Sus scrofa (pig) | Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain |
| 2 | B, E, G, I, K... (F, G, D, I, B...) | Tubulin beta chain | polymer | 445 | 49907.8 | 6 | UniProt (P02554) Pfam (PF00091) Pfam (PF03953) | Sus scrofa (pig) | Beta-tubulin |
| 3 | C, L (N, M) | Microtubule-associated protein RP/EB family member 3 | polymer | 203 | 22865.2 | 2 | UniProt (Q9UPY8) Pfam (PF00307) | Homo sapiens (human) | EB1 protein family member 3, EBF3, End-binding protein 3, EB3, RP3 |
| 4 | AA, DA, O, R, U... (A, K, E, J, C...) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 6 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 5 | BA, EA, P, S, V... (A, K, E, J, C...) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 6 | CA, FA, Q, T, W... (B, H, F, G, D...) | 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE | non-polymer | 539.2 | 6 | Chemie (GSP) PubChem (135398675) PubChem (135476260) PubChem (136073095) PubChem (136440011) PubChem (137176535) PubChem (169450542) |
Sequence modifications
N, M: 1 - 200 (UniProt: Q9UPY8)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 646403.7 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 6520.4 | |
| All* | Total formula weight | 652924.1 |
