3JAK
Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 (merged dataset with and without kinesin bound)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D, F, H, J... (E, J, C, L, A...) | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 6 | UniProt (Q2XVP4) Pfam (PF00091) Pfam (PF03953) | Sus scrofa (pig) | Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain |
| 2 | B, E, G, I, K... (F, G, D, I, B...) | Tubulin beta chain | polymer | 445 | 49907.8 | 6 | UniProt (P02554) Pfam (PF00091) Pfam (PF03953) | Sus scrofa (pig) | Beta-tubulin |
| 3 | C, L (N, M) | Microtubule-associated protein RP/EB family member 3 | polymer | 203 | 22865.2 | 2 | UniProt (Q9UPY8) Pfam (PF00307) | Homo sapiens (human) | EB1 protein family member 3, EBF3, End-binding protein 3, EB3, RP3 |
| 4 | AA, DA, O, R, U... (A, K, E, J, C...) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 6 | Chemie (GTP) | |||
| 5 | BA, EA, P, S, V... (A, K, E, J, C...) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
| 6 | CA, FA, Q, T, W... (B, H, F, G, D...) | 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE | non-polymer | 539.2 | 6 | Chemie (GSP) |
Sequence modifications
N, M: 1 - 200 (UniProt: Q9UPY8)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 646403.7 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 6520.4 | |
| All* | Total formula weight | 652924.1 |
