3JAK
Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 (merged dataset with and without kinesin bound)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
| Method | Blot once for 4 seconds before plunging. |
EM Data Collection
| Microscope | FEI TITAN |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 (4k x 4k) |
| Number of Images | 345 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.3 |
| Software | EMAN,FREALIGN |
